This study is entitled “An Analysis Of Moral Values In “Charlotte's Web” Novel by E.B. White”. The study is aimed at describing the moral values and the significance of moral values in life portrayed in “Charlotte's Web” novel. The research method used in this study is descriptive analysis method.Method of collecting data is the documentation method. The data of this study were analyzed by following steps : content analysis, synthesis and also interpreting, then analyzing kinds of moral values and the significance of moral values in “Charlotte's Web” novel. After analyzing the moral values, it can be concluded that “Charlotte's Web” novel by E.B. White concerns with some parts of human’s life true experience those are never give up, perseverance, responsible, love, loyalty, friendship and appreciating diversity. Amongst the significances of Moral Values in human’s life portrayed in “Charlotte's Web” novel are hard work pays off, realizing the importance of true friends, staying humble, keeping the promise and being tolerant or appreciating diversity.